Fig. 6.

Sustained pUS28 expression is required to maintain viral latency. (A) Kasumi-3 cells (moi = 1.0) or (B) CD34⁺ HPCs (moi = 2.0) were infected with WT (blue), US28Δ (green), or US28comp (gray). (A and B) Cells were collected at the indicated time points and reactivation events were determined by ELDA. Data in A are presented as fold change in virus release relative to WT virus on day 3. Data in B are presented as fold change in virus release relative to WT virus. In all cases, error bars represent SDs of three biological replicates. Statistical significance was calculated relative to WT at the same time point using (A) two-way ANOVA analysis or (B) one-way ANOVA and Tukey post hoc analysis; *P < 0.05, **P < 0.01, ***P < 0.001.