Fig. 1.
Mitochondrial respiration and associated protein abundance in the last third of pregnancy. (A and B) Oxygen consumption in LEAK and OXPHOS states using (A) Py and (B) Pal, (C) RCRs for Py and Pal, and (D) OXPHOS respiration in the presence of malate, glutamate, and succinate (GMSP) in the placental Jz and Lz, as well as (E and F) protein abundance in the (E) Jz and (F) Lz at D14, D16, and D19 of pregnancy. Analyzed by two-way ANOVA (age and zone) with Bonferroni post hoc tests. Different letters represent a significant difference between gestational ages, within a zone (P < 0.05); * denotes a significant difference of Lz to Jz, for a given age (P < 0.05). Outcome of ANOVA is shown if pair-wise comparisons were not significant; n = 6 to 10 per age for A–D, and n = 5 to 6 for E and F per treatment. ATPase, ATP synthase; CI−IV, ETS complexes I to IV.