Fig. 4.
Mice lacking TRPM2 exhibit enhanced Cdk5-specific phosphorylation and synaptic properties. (A) Representative immunoblots of the lysates from mice hippocampal primary neurons treated with roscovitine (50 μM) for 12 h (n = 3 per group). (B) Representative immunoblots of the hippocampal DG lysates (n = 3 per group). (C) Representative traces of mEPSCs recorded from slices of DG neurons of Trpm2+/+ and Trpm2−/− mice under home cage (Left) and CUS conditions (Right), respectively. (D) Cumulative probability plots of the peak amplitudes of mEPSC recordings from Trpm2+/+ and Trpm2−/− DG neurons (n = 3–4 mice per group). (E) Cumulative probability plots of the interevent intervals of mEPSC recordings from Trpm2+/+ and Trpm2−/− DG neurons (n = 3–4 mice per group). Data are means ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001. Other statistical parameters are listed in SI Appendix, Table S1.