Figure 2.

Effect of PrP^C silencing on Akt and ERK phosphorylation induced by recPrP^C. hDPSCs, untreated or treated with 0.5 µg/mL of recPrP^C for 10 min in presence or in absence of pre-treatment with siRNA PrP or scrambled siRNA for 72 h, were analyzed by Western blot using anti-pAkt, anti-total Akt (A). anti-pERK1/2 and anti-total ERK1/2 (B). Densitometric analysis is shown in the right panel. Results represent the mean ± SD from 3 independent experiments, * p recPrP^C treated cells <0.01 vs. untreated cells, ** p siRNA PrP + recPrP^C treated cells vs. scrambled + recPrP^C treated cells. As control, scrambled siRNA was employed in each experiment.