Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 16;20(2):345. doi: 10.3390/ijms20020345

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effect of recPrP^C on expression of neuronal markers GAP-43, NFH and B3-Tubulin. hDPSCs, untreated or treated with recPrP^C for 14 days, were analyzed by flow cytometry and immunofluorescence analysis using anti-NFH, Anti-B3-Tubulin and anti-GAP-43. (A) Flow Cytometry. Histograms represent log fluorescence vs. cell number, gated on cell population of a side scatter/forward scatter (SS/FS) histogram. Cell number is indicated on the y-axis and fluorescence intensity is represented on the x-axis. Each panel was compared with the corresponding secondary antibody as negative control. A representative experiment among 3 is shown. (B) Immunofluorescence analysis. Alternatively, hDPSCs were analyzed by immunofluorescence analysis using anti-B3-Tubulin, anti-NFH and anti-GAP-43 and observed with a Zeiss Axio Vert. A1 fluorescence microscope (Zeiss, Oberkochen, Germany). Scale bars, 100 µm.