Phylogenetic analysis and gene structure of the rice trihelix family. (A) Phylogenetic analysis of the rice trihelix family. The phylogenetic tree was constructed based on the full-length amino acid sequences of the rice trihelix proteins by using MEGA v. 7.0 with the maximum-likelihood method. Bootstrap = 1,000. SIP1, GTγ, GT, SH4, and GTδ are marked with different colors. (B) Gene structures of the rice trihelix family. These were analyzed by the Gene Structure Display Server (GSDS v. 2.0). Exons, introns, and untranslated regions are marked by round red rectangles, black lines, and blue rectangles, respectively. The scale bar at the bottom estimates the lengths of the exons, introns, and untranslated regions.