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. 2019 Jan 9;20(2):241. doi: 10.3390/ijms20020241

Figure 6.

Figure 6

The inhibitory effect of dexamethasone (DEX) released from liposomes on tBH-induced angiogenesis. (A) Schematic representation of the experimental setup. (B) Dexamethasone-loaded liposome drug release profile, showing sustained drug release. (C) ELISA assay showing secretion of VEGF by hiPSC-RPE after stimulation with tBH. The values are the means from three independent measurements expressed as a percentage relative to the control with SD error bars. (D) The tube formation assay on HUVECs after stimulation with RPE-conditioned media as shown in (A). Representative bight-field images shown at the top, quantification of total tube length (left) and total segment length (right)—at the bottom. Original magnification, × 20. In (C,D), control—untreated HUVECs, tBH—HUVECs treated with tBH, tBH/DEX—HUVECs pretreated with tBH followed by exposure to an insert with the dexamethasone-loaded liposomes. The values in (B–D) are the means from three independent measurements with SD error bars. * p < 0.05 Student’s t-test, vs. control.