Figure 5.

Incubation of HepG2 cells with 10 µM 5-Azacytidine plus 0.5 mM Vitamin C (dark bars) and in the presence of insulin plus hydrocortisone (black bars) increased basal Cytochrome P450 (CYP) gene expression. The gene expression of CYP1A2, 3A4 and CYP2C9 were measured by qRT-PCR after 48 h of incubation. Unstimulated controls (grey bars) as well as primary human hepatocytes (PHH) (red line) were also cultured for 48 h. The values were normalized to GAPDH. Data represent the mean of N = 3, n = 3. As positive reference served a pool of five different PHH donors.