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. 2019 Jan 16;20(2):347. doi: 10.3390/ijms20020347

Table 4.

Substrates, concentrations, conditions and measured reactions of the CYP activity measurement.

Substrate Isoenzyme Incubation Time in Hours Concentration Reaction
Bupropion CYP2B6 1 100 μM Bupropion-hydroxylation
Diclofenac CYP2C9 1 9 μM Diclofenac-4’-hydroxylation
Testosterone CYP3A4 1 50 μM Testosterone-6β-hydroxylation
Bufuralol CYP2D6 2 9 μM Bufuralol-1-hydroxylation