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MUC1 was enriched in the exosomes of NCI-H838 cell line. Western blotting analysis was performed to measure expression of MUC1 in exosome, membrane, cytosolic and whole cell protein of NCI-H838 cells. 1 μg of each type of protein was separated by SDS-PAGE and 16A [41] monoclonal antibody was used as primary antibody for western blot.
