Figure 10.

VEGF gene silencing using liposome-siRNA complexes in HeLa cells. Cells were treated with complexes for 4 h at 37 °C or 42 °C followed by incubation for 44 h at 37 °C. VEGF mRNA levels were estimated by real time-PCR normalized to β-actin mRNA. The relative expression of VEGF was estimated by the VEGF expression level of HeLa cells at 37 °C as a control. The error bars indicate the range of the relative expression of VEGF using real time PCR. The dotted line indicates the difference in incubation temperature.