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. 2019 Jan 14;20(2):320. doi: 10.3390/ijms20020320

Figure 2.

Figure 2

Effect of PF-04217903 and Src Inhibitor-1 on HGF-dependent NT2D1 cell chemotaxis. All experiments were performed at least in quadruplicate. (I) Effect of c-MET inhibitor (PF-04217903). Left panel: Graphical representation of the amount of chemo-attracted NT2D1 cells. HGF significantly increases cell migration. C-MET inhibitor (PF-04217903) administration abrogates the migratory effect induced by HGF, even if this drug is not able to affect NT2D1 cell migration when administered alone. The values of treated samples were reported as fold-change compared with control values (arbitrarily considered as 1) (b vs. a p < 0.001). Right panel: Representative bright-field microscopy images (40 × magnification) of the filters with the NT2D1 migrated cells. Lower panel: Table illustrating the real number of migrating cells/filter in all the experimental conditions reported in the respective “fold-change” graph (b vs. a p < 0.001). (II) Effect of Src inhibitor-1. Left panel: Graphical representation of the amount of chemo-attracted NT2D1 cells. Src inhibitor-1 abrogates the chemotactic effect induced by HGF, even if this drug is not able to affect NT2D1 cell migration when administered alone. The values of treated samples were reported as fold-change compared with control values (arbitrarily considered as 1) (b vs. a p < 0.001). Right panel: Representative bright-field microscopy images (40 × magnification) of the filters with the NT2D1 migrated cells. Lower panel: Table illustrating the real number of migrating cells/filter in all the experimental conditions reported in the respective “fold change” graph (b vs. a p < 0.001).