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. 2019 Jan 14;20(2):320. doi: 10.3390/ijms20020320

Figure 5.

Figure 5

Effect of Src Inhibitor-1 on NT2D1 cell collective migration: Wound-healing assay on NT2D1 cells cultured in DMEM + 2% FBS alone (CTRL), or added with HGF, Src inhibitor-1 or HGF+Src inhibitor-1. Four inserts were used for each experimental condition in each experiment. The experiment was performed in triplicate. (I) Representative phase-contrast images of wound-healing assay taken immediately after insert removal (T0) for wound gap (dotted red lines) measurement, or taken 24 h and 48 h after wounding. Images were photographed at 10 × magnification (scale bar: 100 µm). (II) Quantitative analysis of wound closure after 24 h (A) and 48 h (B) from insert removal. Data are expressed as the mean percentage of open residual area compared with the respective cell-free gap at T0. After 24 h the difference of open residual area between CTRL and HGF treated wells was not statistically significant. However, after 48 h of culture the decrease of open area in HGF treated wells was statistically significant with respect to the control wells (* p < 0.001). Src inhibitor-1 in combination with HGF both at 24 h (# p < 0.01) and 48 h (# p < 0.001) abrogated the migratory effect induced by HGF. Src inhibitor-1 alone was also able to inhibit the basal collective migration of the NT2D1 cells, compared with control wells, both at 24 h ($ p < 0.05), and 48 h ($ p < 0.001) after insert removal.