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. 2019 Jan 14;20(2):320. doi: 10.3390/ijms20020320

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Confocal microscopy analysis of c-Src phospho Tyr416 distribution-pattern: Immunofluorescence analyses of c-Src phospho Tyr416 (FITC, green signal) in NT2D1 cells cultured with or without HGF and Src Inhibitor-1. Actin cytoskeleton (Rhodamin phalloidin, red signal) and nuclei staining (TOPRO-3, blue signal) are also reported in the figure. The nuclear translocation of c-Src phospho Tyr416 after HGF administration is clearly evident. The treatment with Src Inhibitor-1 prevents this phenomenon.