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. 2019 Jan 12;20(2):294. doi: 10.3390/ijms20020294

Figure 8.

Figure 8

Direct effect of ginsenoside Rb1 and diarylpropionitrile (DPN) on ER-β activation in 293T cells. ER-β cDNA plasmid and ERE-Firefly luciferase reporter plasmid as well as Renilla luciferase reporter plasmid were co-transfected into 293T cells. ERE-Firefly Luciferase activities were normalized with internal control of Renilla luciferase activities. Ginsenoside Rb1 or/and DPN activated ER-β and reporter gene expression in 293T cells, indicating that both Rb1 and DPN can directly activate ER-β for a genomic response. ER-β activation data were standardized with the untreated control group as 1. Student’s t-test was used to compare the control with the treated group or between two groups. * p < 0.05. n = 3/group. ER-β (estrogen receptor β); DPN (specific ER-β activator); ERE (estrogen responsive element); 293T cells (cell line derived from human embryonic kidney cells).