Figure 3.

Viability of Caco-2 intestinal cells incubated for 48 h with ascorbic acid and tocopherol in aqueous solution or co-loaded in liposomes, nutriosomes and EU-nutriosomes (A). Data are reported as mean values ± standard deviations of cell viability expressed as the percentage of control cells (100% viability). Antioxidant activity (AA%) of ascorbic acid and tocopherol in aqueous solution or co-loaded in liposomes, nutriosomes and EU-nutriosomes, according to the DPPH assay (B). Viability of Caco-2 cells exposed to hydrogen peroxide in the absence or presence of ascorbic acid and tocopherol in aqueous solution or co-loaded in liposomes, nutriosomes and EU-nutriosomes (C). Data are reported as mean values ± standard deviation of cell viability expressed as the percentage of the negative control (100% viability). *^,+ symbols indicate statistically different samples (p < 0.05) vs. hydrogen peroxide-treated cells.