Table A1.

The selected significant GO terms assigned with AgriGO, gProfiler and ATTED-II to 78 genes of the physiological adaptation to spaceflight in WT cells, and 221 genes of the physiological adaptation to spaceflight in HSFA2 KO cells. In some instances, the gene duplicates within ontology were removed and assigned to the most specific available GO term class. Cells highlighted in red indicate upregulation; in green indicate downregulation. Cells marked with “x” indicate no statistically significant differential expression.

ONT	GO Term Name
	Transcript ID	Gene Symbol	Gene Description	FWt : GWT FC log2	FHsf : GHsf FC log2
BP	defense response to other organism GO:0098542
	AT2G03760	ST1	sulphotransferase 12	1.1	1.0
	AT5G38980		unknown	3.3	X
	At3g43250		unknown in wounding	2.6	X
	AT4G02460	PMS1	DNA mismatch repair protein, putative	2.2
	AT2G44490	PEN2	Glycosyl hydrolase superfamily protein	1.4	X
	AT1G59124		Disease resistance protein (CC-NBS-LRR class)	X	2.4
	AT2G17430	NTA	Seven transmembrane MLO family protein	X	1.9
	AT4G35770	SEN1	Rhodanese/Cell cycle control phosphatase	X	1.8
	AT3G61060	PP2-A13	phloem protein 2-A13	X	1.5
	AT3G09940	MDHAR	monodehydroascorbate reductase	X	1.4
CC	plant-type cell wall GO:0009505
	AT1G30600		Subtilase family protein	2.5	X
	At4g30500		unknown in cellulose biosynthetic process	2.4	X
	AT2G44490	PEN2	Glycosyl hydrolase superfamily protein	1.4	X
	AT1G78860		curculin-like (mannose-binding) lectin	1.3	X
	AT5G44130	FLA13	FASCICLIN-like arabinogalactan protein 13 precursor	−2.1	X
	AT2G06850	XTH4	xyloglucan endotransglucosylase/hydrolase 4	X	−2.4
	AT5G51550	EXL3	EXORDIUM like 3	X	−2.1
	AT5G13980		Glycosyl hydrolase family 38 protein	X	−1.6
	AT3G45970	EXPL1	expansin-like A1	X	−1.4
	AT1G15390	PDF1A	peptide deformylase 1A	X	−1.4
	AT1G75750	GASA1	GAST1 protein homolog 1	X	−1.4
	AT4G08950	EXO	EXO Exordium, Phosphate-responsive 1 protein	X	−1.1
	AT1G73590	PIN1	Auxin efflux carrier family protein	X	−1.0
BP	regulation of biological process GO:0050789 regulation of metabolic process GO:0019222 regulation of cellular metabolic process GO:0031323
	AT3G62080		SNF7 family protein	2.6	X
	AT5G48560		basic helix-loop-helix (bHLH) DNA-binding	2.4	X
	AT5G01920	STN8	Protein kinase superfamily protein	2.4	X
	AT2G46225	ABIL1	ABI-1-like 1	2.2	X
	AT5G56270	WRKY2	WRKY DNA-binding protein 2	1.9	X
	AT1G27370	SPL10	squamosa promoter binding protein-like 10	1.7	X
	AT5G62710		Leucine-rich repeat protein kinase family protein	1.4	X
	AT5G05130		DNA/RNA helicase protein	1.1	X
	AT5G07580		Integrase-type DNA-binding superfamily protein	−3.3	X
	AT4G21380	RK3	receptor kinase 3	−2.9	X
	AT2G35530	bZIP16	basic region/leucine zipper transcription factor 16	−2.7	X
	AT1G78980	SRF5	STRUBBELIG-receptor family 5	−2.4	X
	AT1G78080	WIND1	related to AP2 4	−1.2	X
	AT4G36530		alpha/beta-Hydrolases superfamily protein	−1.2	X
CC	endomembrane system GO:0012505 Golgi apparatus GO:0005794
	At3g18260		Reticulon family protein	3.1	X
	At1g77510	PDIL1-2	PDI-like 1-2 protein disulfide isomerase-like 1-2	1.2	X
	At2g03760	ST1	sulphotransferase 12	1.1	X
	AT5G19070		SNARE associated Golgi protein family	−2.6	X
	At2g22900		Galactosyl transferase GMA12/MNN10	−2.4	X
	At4g36640		Sec14p-like phosphatidylinositol transferprotein	−1.8	X
	At2g32720	CB5-B	cytochrome B5 isoform B	−1.5	X
	AT1G19970		ER lumen protein retaining receptor protein	−1.4	X
	AT2G43240		Nucleotide-sugar transporter family protein	−1.3	X
BP	cellular response to sucrose starvation GO:0043617
	AT3G06850	DIN3	2-oxoacid dehydrogenases acyltransferases	X	1.2
	AT3G13450	DIN4	branched chain alpha-keto acid dehydrogenase E1 beta	X	1.3
	AT1G21400	E1α	Thiamin diphosphate-binding fold (THDP-binding)	X	1.6
	Valine, leucine and isoleucine degradation GO:0009083
	AT3G06850	DIN3	2-oxoacid dehydrogenases acyltransferase family protein	X	1.2
	AT3G13450	DIN4	branched chain alpha-keto acid dehydrogenase E1 beta	X	1.3
	AT1G21400	E1α	Thiamin diphosphate-binding fold (THDP-binding)	X	1.6
	AT2G14170	ALDH6B2	aldehyde dehydrogenase 6B2	X	1.2
	At4g34030	MCCB	subunit β of methylcrotonyl-CoA carboxylase (MCCB)	X	1.1
CC	plasma membrane GO:0005886
	AT5G67130		PLC-like phosphodiesterases superfamily	X	−2.7
	AT3G21180	ATACA9	autoinhibited Ca(2+)-ATPase 9	X	−2.7
	AT5G65440			X	−1.9
	AT5G15350	ENODL17	early nodulin-like protein 17	X	−1.7
	AT5G13980		Glycosyl hydrolase family 38 protein	X	−1.6
	AT3G54920	PMR6	Pectin lyase-like superfamily protein	X	−1.5
	AT1G17620		Late embryogenesis abundant (LEA)	X	−1.2
	AT5G03700		D-mannose binding lectin protein	X	−1.1
	AT1G73590	PIN1	Auxin efflux carrier family protein	X	−1.0
BP	developmental process GO:0032502 anatomical structure morphogenesis GO:0009653
	AT2G35340	MEE29	helicase domain-containing protein	X	−3.2
	AT3G63290		2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase	X	−3.1
	AT5G65930	ZWI	kinesin-like calmodulin-binding protein (ZWICHEL)	X	−3.0
	AT4G02460	PMS1	DNA mismatch repair protein, putative	X	−2.9
	AT3G47450	RIF1	P-loop containing nucleoside triphosphate hydrolase	X	−2.8
	AT3G21180	ATACA9	autoinhibited Ca2+-ATPase 9	X	−2.7
	AT2G06850	XTH4	xyloglucan endotransglucosylase/hydrolase 4	X	−2.4
	AT4G15570	MAA3	P-loop containing nucleoside triphosphate hydrolase	X	−1.8
	AT3G54220	SCR	SCR Scarecrow alias SGR1 Shoot Gravitropism	X	−1.7
	AT3G45970	EXPL1	expansin-like A1	X	−1.4
	AT1G75750	GASA1	GAST1 protein homolog 1	X	−1.4
	AT4G03190	GRH1	GRR1-like protein 1	X	−1.1
	AT1G73590	PIN1	Auxin efflux carrier family protein	X	−1.0