Table 1.
Primers for PCR amplification of each studied gene.
| Gene | Sense Primer | Anti-Sense Primer | Annealing ta (°C) |
|---|---|---|---|
| atgl | 5′-GAGCTTCGCGTCACCAAC-3′ | 5′-CACATCTCTCGGAGGACCA-3′ | 60.0 |
| hsl | 5′-GGTGACACTCGCAGAAGACAATA-3′ | 5′-GCCGCCGTGCTGTCTCT-3′ | 60.0 |
| lpl | 5′-CAGCTGGGCCTAACTTTGAG-3′ | 5′-CCTCTCTGCAATCACACGAA-3′ | 61.5 |
| fasn | 5′-AGCCCCTCAAGTGCACAGTG-3′ | 5′-TGCCAATGTGTTTTCCCTGA-3′ | 60.0 |
| β-actin | 5′-ACGAGGCCCAGAGCAAGAG-3′ | 5′ -GGTGTGGTGCCAGATCTTCTC-3′ | 60.0 |
| srebf1 | 5´- GCTGTTGGCATCCTGCTATC-3′ | 5′-TAGCTGGAAGTGACGGTGGT-3′ | 60.0 |
| cebpβ | 5′-CAAGCTGAGCGACGAGTACA-3′ | 5′-CAGCTGCTCCACCTTCTTCT-3′ | 67.5 |
| bcl2 | 5′-AGTACCTGAACCGGCATCTG-3′ | 5′-GGGGCCATATAGTTCCACAAA-3′ | 60.0 |
atgl = adipose triglyceride lipase; hsl = hormone sensitive lipase; lpl = lipoprotein lipase; fasn= fatty acid synthase; srebf1 = sterol regulatory element-binding factor 1; cebpβ = CCAAT-enhancer- binding protein β; bcl2 = B cell leukemia/lymphoma 2.