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. 2019 Jan 16;20(2):363. doi: 10.3390/ijms20020363

Figure 4.

Figure 4

Effect of AZD1208 on expression and phosphorylation of mTOR, S6 and eIF-2α in 93T449 cells. (A) 93T449 cells were treated with AZD1208 (20 µM) or vehicle control (DMSO) for the times designated. At each time point, whole cell lysates were prepared and analyzed for p-mTOR and T-mTOR by Western blotting. p-mTOR, phosphorylated mTOR; T-mTOR, total mTOR. (B) Western blotting analysis in triplicate experiments for 24 h. (C) The densitometry data of (B). * p < 0.05 compared to the control at the indicated time. (D) 93T449 cells were treated with AZD1208 (20 µM) or vehicle control (DMSO) in triplicate experiments for indicated times. At each time point, whole cell lysates were prepared and analyzed for p-S6, T-S6, p-eIF-2α and T-eIF-2α by Western blotting. p-S6, phosphorylated S6; T-S6, total S6; p-eIF-2α, phosphorylated eIF-2α; T-eIF-2α, total eIF-2α. (E) The densitometry data of (D). * p < 0.05 compared to the control at the indicated time.