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. 2019 Jan 16;20(2):363. doi: 10.3390/ijms20020363

Figure 6.

Figure 6

Effect of AZD1208 and/or knock-down of LKB-1 on expression and phosphorylation of LKB-1 and AMPK and cellular ATP content in 93T449 cells. (A) 93T449 cells were treated with AZD1208 (20 µM) or vehicle control (DMSO) for the times designated. At each time point, whole cell lysates were prepared and analyzed for p-LKB-1 and T-LKB-1 by Western blotting. p-LKB-1, phosphorylated LKB-1; T-LKB-1, total LKB-1. The image is a representative of three independent experiments. (B) 93T449 cells were transfected with 100 pM of control siRNA (siCon) or LKB-1 siRNA (siLKB-1) for 24 h. The siCon- or siAMPK-transfected cells were treated with AZD1208 (20 µM) or vehicle control for 24 h. Whole cell lysates were prepared and analyzed for T-LKB-1, p-LKB-1, p-AMPK and T-AMPK by Western blotting. (C) 93T449 cells were treated with AZD1208 (20 µM) or vehicle control for the times designated. For comparison, cells were treated with 1 mM of deoxyglucose (2-DG), a known ATP depleting agent for 24 h. At each time point, cellular ATP content was measured by ATP measurement kit. * p < 0.05 compared to the value of AZD1208 or 2-DG free control at the indicated time.