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Circular dichroism (CD) spectra of siRNA Bcl-xL (2 µM) in the presence of the carbosilane dendrimers of (a) the second ([BDEF32] = 15 µM) and (b) the third generations ([BDEF33] = 6 µM); (c) changes of ellipticity (at 260 nm) of the dendriplexes formed by siRNA Bcl-xL and dendrimers BDEF32 and BDEF33 (# marks samples after heparin treatment). Conditions: 1×PBS (10 mM phosphate buffer, pH 7.4, 137 mM NaCl, 2.7 mM KCl), 25 °C.
