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Biosynthesis of H2S in the kidney. The biological production of H2S in the kidney is mediated by four pathways. CSE and CBS can use l-cysteine and homocysteine as substrates to generate H2S in cytosol, while they can translocate into mitochondria in hypoxic states. l-cysteine and d-cysteine have to be catalyzed into 3MP before they can be utilized by 3-MST for the production of H2S. 3-MST mediates H2S production, mainly in mitochondria.
