Table 4.

Kinetic analysis of compounds, 3a, 4a, 3b, and 4b.

Compounds	Dose (µM)	1/Vmax (ΔA/Sec)	Km (µM)	Inhibition Type	Ki (µM)	Kiʹ (µM)
3a	0.0	12	2.5	Non-competitive	0.6	---
	0.25	20	2.5
	0.50	30	2.5
	1.0	35	2.5
	2.0	44	2.5
4a	0.0	8	0.47	Non-competitive	2.1	---
	0.75	12	0.47
	1.5	18	0.47
	3.0	22	0.47
	6.0	35	0.47
3b	0.0	50	7.14	Mixed-inhibition	1.2	3.0
	0.25	60	10
	0.5	65	12.5
	1.0	70	14.2
	2.0	80	16.6
4b	0.0	55	0.41	Non-competitive	2.4	---
	1.0	65	0.41
	2.0	100	0.41
	4.0	130	0.41
	6.0	180	0.41

V_max is the reaction velocity; K_m is the Michaelis-Menten constant; Ki is the EI dissociation constant; Kiʹ is the ESI dissociation constant; ---: not determined