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. 2019 Jan 18;20(2):405. doi: 10.3390/ijms20020405

Figure 2.

Figure 2

Cell viability, TF expression at mRNA and protein levels in HUVEC after treated with different concentration of peach kernel oil. No chemicals were added in the normal group. As a vehicle control, DMSO was added for evaluating the solvent effect. For the model group, TNF-α was added to the culture medium to stimulate TF protein expression. For the positive group, simvastatin was added to HUVEC to suppress the TF protein induction by TNF-α stimulation. After pretreating HUVEC with simvastatin or peach kernel oil, TNF-α protein was added to HUVEC culture medium to induce TF protein expression. (A) Cell viability of HUVEC after treated with different concentrations of peach kernel oil. The TF expression at mRNA (B) or protein (C) level in HUVEC after treated with different concentrations of peach kernel oil. (D–L) The expression of TF protein in HUVEC after treated with peach kernel oil detected by immunohistochemistry (yellow color). The cell nuclei were counterstained with DAPI. PKO stands for peach kernel oil. Different labels above columns in (A–C) indicate a significant difference tested by One-way ANOVA between different experimental groups with p < 0.01.