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. 2019 Jan 19;20(2):428. doi: 10.3390/ijms20020428

Figure 1.

Figure 1

Cytokine production by CD4+ T cell clones derived from decidua of those experiencing successful pregnancy and URA and mRNA expression of cytokines and transcription factors in decidual biopsies of successful pregnancy. CD4+ T cell clones were generated from decidual biopsies, and peripheral blood was obtained from those experiencing successful pregnancy and those experiencing unexplained recurrent abortion (URA) (Experiment 1 in Section 4.3). IL-4, IL-13, IL-5, IL-17A, IL-17F, IL-22, and IFN-γ were measured in the supernatant of the CD4+ T cell clones by a multiplex bead-based assay. The statistical analysis was performed with the Wilcoxon test. The determination of mRNA level for IL-4, GATA-3, IL-17A, ROR-C, IL-22, AHR, T-bet, and IFN-γ in three biopsies of decidua from three pregnant women (with successful pregnancy) was performed by Quantigene 2.0.