Figure 1.

Cytotoxic activity of E. menziesii’s extracts over Artemia salina brine shrimp. (A) Percentage of brine shrimp death at 5, 50 or 500 μg/mL of hexane (Hx), chloroform (Chl) or methanol (MeOH) extracts obtained from E. menziesii seaweed for 24 h. l-Ascorbic acid (LAA) and artificial seawater were used as positive and negative controls, respectively. Data are expressed as the mean ± SEM of at least three independent experiments. Statistical differences were determined by two-way ANOVA, and differences among groups by Tukey’s test with multiple comparisons. a–g nomenclature: the same lowercase letters on each bar mean there are not differences among treatment values; conversely, distinct letters indicate significant differences within treatments (p < 0.01). (B) Half maximal lethal concentration (LC₅₀) of Hx, Chl and MeOH extracts, and LAA was determined by linear regression.