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(a) Genetic encoding of the nitroxide ncAA 6. (b) Rotamers of 6 attached to thioredoxin (pdb id 2TRX). (c) Distance distributions resulting from DEER experiment (green) or rotamer simulation (blue). (b+c) Reprinted with permission from EPR Distance Measurements in Native Proteins with Genetically Encoded Spin Labels, M. Schmidt, et al., ACS ChemBiol 2015 10 (12), 2764–2771 [55]. Copyright (2015) American Chemical Society.
