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. 2019 Jan 9;9(1):10. doi: 10.3390/metabo9010010

Figure 2.

Figure 2

Screen for ASNase Activity Quenchers. The following conditions were tested for the ability to quench the conversion of Asn to Asp by ASNase: (1) 80:20 methanol (MeOH):water; (2) 80:20 MeOH containing 1% formic acid (FA):water; (3) 80:20 acetonitrile (ACN):water; (4) 80:20 ACN containing 1% FA:water; (5) 80:20 water containing 1% FA:water; (6) 80:20 water containing 10% FA:water; (7) 80:20 water containing 10% (w/v) sulfosalicylic acid (SSA):water; (8) 80:20 water containing 1% (w/v) trichloroacetic acid (TCA):water; (9) 80:20 water containing 40 mM 5-diazo-4-oxo-l-norvaline (DONV):water; (10) positive control sample containing ASNase in water; and (11) negative control sample containing water without ASNase. Data are presented as normalized response of Asn and Asp. The final activity of ASNase and solution concentration of Asn in each sample tested was approximately 20 IU/mL and 100 µM, respectively.