Table 2.
Inter-day mean concentration, accuracy, and precision for QC standards prepared in dialyzed whole blood matrix.
| [Asn]nominal (µM) | [Asn]mean (µM) a | Accuracy (%RE) b | Precision (%CV) c |
|---|---|---|---|
| 12.0 | 10.6 | −11.8% | 4.78% |
| 200 | 182 | −9.00% | 2.31% |
| 3200 | 3140 | −1.89% | 2.38% |
| [Asp]nominal (µM) | [Asp]mean (µM) a | Accuracy (%RE) b | Precision (%CV) c |
| 12.0 | 14.6 | 21.9% | 7.05% |
| 200 | 214 | 7.16% | 3.75% |
| 3200 | 3155 | 1.41% | 2.57% |
| [Gln]nominal (µM) | [Gln]mean (µM) a | Accuracy (%RE) b | Precision (%CV) c |
| 12.0 | 11.1 | −7.87% | 5.77% |
| 200 | 215 | 7.60% | 2.32% |
| 3200 | 3675 | 14.8% | 2.50% |
| [Glu]nominal (µM) | [Glu]mean (µM) a | Accuracy (%RE) b | Precision (%CV) c |
| 12.0 | 14.4 | 20.0% | 6.13% |
| 200 | 209 | 4.36% | 2.16% |
| 3200 | 3122 | −2.42% | 1.56% |
a The mean concentration was calculated from 1/x weighted linear least-squares regressions from the individual calibration curves in each batch (n = 5 over five non-consecutive days) after correcting for the endogenous amino acid content contained in the dialyzed whole blood matrix; b percent relative error: %RE = (([AA]mean/[AA]nominal)-1) * 100; c coefficient of variation.