Table 2.
Spatial expression pattern of LjPIN genes in relation to the root nodule developmental stage. “+,” signal; “−”, no signal; “w,” signal weak; “s,” signal sectorial.
| Nodule Developmental Stage | Expression Pattern of LjPIN Genes | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| LjPIN1 | LjPIN2 | LjPIN3 | LjPIN4 | LjPIN5 | LjPIN6 | LjPIN7 | LjPIN8 | ||
| Active initial primordia | − | + | + | + | w | + | + | + | |
| “Hidden” primordia (tissues not differentiated) | + | + | + | + | − | + | + | w | |
| “Emerged” primordia (tissues discernible, nondifferentiated) | Vascular bundle | + | + | − | + | + | + | + | w |
| Cortex | − | + | s | + | − | + | − | w | |
| Vascular bundle–stele connection | + | + | + | + | + | + | + | + | |
| Nodules 20 dpi (young, tissues differentiated) | Vascular bundle | + | − | − | s | + | + | s | − |
| Cortex | − | − | − | − | − | − | − | − | |
| Vascular bundle–stele connection | + | − | + | + | + | + | + | + | |
| Nodules 54 dpi (mature) | Vascular bundle | w | ws | − | w | − | s | − | − |
| Cortex | − | − | − | − | − | − | − | − | |
| Vascular bundle–stele connection | w | w | − | w | + | + | − | + | |