Table 1.

Biosensor kinetic parameters obtained after different storage conditions. Four groups of biosensors (n = 4 per design) were stored at −80 °C for 28 days and calibrated immediately after stabilization in PBS at RT. Further four groups of biosensors were used for simulating the transport in dry ice by storing the biosensors at −70 °C for 48 h after a period of 26 days at −80 °C. * = p < 0.05 vs. the corresponding biosensor group stored at −80 °C for 28 days.

Biosensor Design	VMAX (nA)		KM (mM)		LRS (nA mM−1)
	(28 days at −80 °C)	(26 days at −80 °C plus 48 h at −70 °C)	(28 days at −80 °C)	(26 days at −80 °C plus 48 h at −70 °C)	(28 days at −80 °C)	(26 days at −80 °C plus 48 h at −70 °C)
GB1	529.03 ± 26.21	487.10 ± 33.5	5.33 ± 0.31	5.74 ± 0.41	61.24 ± 2.11	57.81 ± 3.61
GB2	592.01 ± 47.90	523.51 ± 52.7	2.42 ± 0.19	3.35 ± 0.33	132.11 ± 6.80	109.10 ± 8.91
LB1	168.02 ± 8.60	139.11 ± 10.20	4.89 ± 0.63	6.20 ± 0.86	32.24 ± 1.35	23.61 ± 3.21
LB2	26.30 ± 0.69	18.50 ± 2.42 *	0.79 ± 0.06	1.74 ± 0.12 *	22.72 ± 0.78	13.50 ± 1.31 *