Table 1.

The procedure of CUMS.

Monday
9:00	Closed light
11:00	Remove food and water, 20 h cold–wet cage (200 mL water (4 °C)/cage)
Tuesday
9:00	Change dry cage, restore food and water, and 40 min of cage shaking (200 rpm)
9:40	Stop cage shaking, continuous light for 24 h
Wednesday
9:00	Closed light, record animal weight
10:00	24 h of tilted cage (45°), and remove water
Thursday
9:00	Stop tilted cage (45°), restore water, and change to 5 mice/cage
15:00	Change to single cage, remove food
Friday
9:00	Restore food, 40 min of cage shaking (200 rpm)
9:40	Stop cage shaking, 20 h hot–wet cage (200 mL water (45 °C)/cage)
Saturday
9:00	Change dry cage
10:00	24 h of tilted cage (45°), and remove water
Sunday
9:00	Stop tilted cage (45°), restore water, and change to 5 mice/cage
15:00	Change to single cage, continuous light for 20 h