Figure 1.

Impact of the loss of retromer function on seed germination and longevity. (a) Germination curve of Col-0 and vps29 Arabidopsis seeds after cold stratification treatment (5 days at 4 °C in the dark) and subsequent transferral to continuous light at 25 °C. Seed germination (considered as radicle protrusion through the seed coat and the endosperm) was scored daily. White circles represent the germination means for Col-0 seeds. Black circles represent the germination means for vps29 seeds. The bars represent the standard deviation (±SD) of the mean of three independent experiments. (b) Influence of controlled deterioration treatment (CDT) on Arabidopsis seed germination. Col-0 and vps29 seeds were submitted to CDT for different periods of time (0, 1, 3, and 5 days). The graph shows the maximum percentage of germination (Gmax) of deteriorated seeds. It is a representative experiment carried out three times in triplicate. The bars represent the standard deviation (±SD) of the mean of three independent experiments. Dashed lines represent the polynomial curve-fit related to these following equations: Col-0, y = −2.3054x² + 5.4464x + 90.901 with R² = 0.99037; vps29, y = 1.5537x² − 26.453x + 107.67 with R² = 0.98204.