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. 2019 Jan 21;20(2):445. doi: 10.3390/ijms20020445

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Building a model of OSA-like intermittent hypoxia. (A) The model for exposing cells to intermittent hypoxia. A programmable gas blender controls oxygen delivery to a small chamber containing cells grown on oxygen permeable dishes. (B) Pericellular oxygen levels during the administered intermittent hypoxia protocol. (C) The peak and nadir pericellular oxygen measurements using different lengths of normoxia and hypoxia in the gas phase (normoxia set to 12% oxygen and hypoxia set to 0% oxygen). Average values ± range.