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. 2019 Jan 11;24(2):264. doi: 10.3390/molecules24020264

Figure 1.

Figure 1

Influences of microRNA-29a (miR-29a) on the expressions of cannabinoid receptor type 1 (CB1R) and profibrotic genes in vitro and in glomeruli of diabetic mice. (A) Effects of high glucose (HG) and miR-29a precursor on CB1R expression in cultured renal mesangial cells by quantitative RT-PCR analysis. (B) Western blot analysis of CB1R protein in mesangial cells. Relative expression levels of CB1R protein quantified by densitometry are shown in the bottom panel. All experimental results from quantitative RT-PCR or Western blotting are presented as means ± standard error of the mean (SEM) calculated from three independent experiments. The symbol * indicates significant difference vs. the control group (p < 0.05); the symbol # indicates significant difference vs. the HG group (p < 0.05). (C) Isolation of glomerular components in renal tissues by laser capture microdissection (LCM). (D) mRNA expression of miR-29a in diabetic (left panel) and transgenic mice (right panel). (E) The mRNA expression levels of CB1R, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), normalized to that of β-actin, in glomeruli of wild-type normal control (WT NC), wild-type diabetic (WT DM), miR-29a transgenic normal control (Tg NC), and miR-29a transgenic diabetic mice were quantified by qRT-PCR (n = 6). The symbol * indicates significant difference vs. the WT NC group (p < 0.05); the symbol # indicates significant difference vs. the WT DM group (p < 0.05).