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. 2019 Feb 1;11:17. doi: 10.1186/s13148-018-0603-z

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Pyrosequencing to identify methylated CpG sites in TLR1. DNA methylation levels at each CpG site (POS) by percentage were analyzed by pyrosequencing in NTDs (black bar) and controls (gray bar) in cg02016764. No significant difference was found between NTDs and controls