Fig. 4.

Reduction of nitric oxide (NO) release from stimulated human keratinocytes by PBP10-containing nanostructures. Release of NO from HaCaT cells stimulated with 1 µg/mL LPS (a), LTA (b) or heat-inactivated suspension of E. coli RS218 (c) or S. aureus A1 (d) and treated simultaneously for 24 h with indicated peptides and their magnetic derivatives at concentrations of 2 µg/mL (dark grey columns), 5 µg/mL (grey columns) and 10 µg/mL (light grey columns). Untreated stimulated control samples are indicated as 100%. Results are presented as mean ± SD obtained from 3 experiments. *Indicates statistically significant (p ≤ 0.05) activity of tested agents compared to untreated control samples, ^{^}Indicates statistical significance when comparing MNP-based agents to non-immobilized peptides