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. 2019 Feb 2;19(1):15. doi: 10.1093/jisesa/iey134

Fig. 4.

Fig. 4.

Prokaryotic expression of recombinant proteins Bmp38 (A) and BmS6K (C) on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) with isopropyl β-D-thiogalactoside. M: protein marker, empty plasmid pET-28a, was used as negative control. Lane 1, target recombinant protein. Recombinant protein purification of Bmp38 (B) and BmS6K (D) on SDS–PAGE. M: protein maker, Lane 1: effluent liquid, Lane 2: washing liquid, and Lane 3–6: elution liquid (contain 250 mM imidazole).