Table 1.
Primers | Sequences of primers (5ʹ–3ʹ) | Purpose |
---|---|---|
Bmp38-F | TCGGCTGGCATCATTCATAG | RT-qPCR |
Bmp38-R | TTCAGTCTCAGTGGGTCTCGC | RT-qPCR |
BmS6K-F | AACCACAATACGCAGAACCCA | RT-qPCR |
BmS6K-R | TCGCACCACCGAGAATGAA | RT-qPCR |
BmGAPDH-F | CATTCCGCGTCCCTGTTGCTAAT | RT-qPCR |
BmGAPDH-R | GCTGCCTCCTTGACCTTTTGC | RT-qPCR |
Bmp38-F | CGCGGATCC*CTTACACCTGTAGGTTCCGG (EcoRI) | Protein expression |
Bmp38-R | CCGCTCGAGTTCTGCCGTTATGCGTTTA (XhoI) | Protein expression |
BmS6K-F | CGCGGATCCGCATTCCAAACAGCTGGTA (EcoRI) | Protein expression |
BmS6K-R | CCGCTCGAGGTCTCTTGGCGTTCTACAG (XhoI) | Protein expression |
GP64-F | GAAGTAGAAACGCCGCATCG | RT-qPCR |
GP64-R | GTGGGGTATCAGGCAGCAGT | RT-qPCR |
GP41-F | CGTAGTAGTAGTAATCGCCGC | RT-qPCR |
GP41-R | AGTCGAGTCGCGTCGCTTT | RT-qPCR |
IE-1-F | AGAAGGAGGACGGCAGCAT | RT-qPCR |
IE-1-R | TCTCGCCAGAAATCCAATAAAAC | RT-qPCR |
*The underline represented restriction enzyme cutting site. RT-qPCR (real-time quantitative polymerase chain reaction).