Minimum bactericidal concentration (MBC) was performed with a dose response of H₂O₂ prepared in saline and in water and compared with a 3% topical solution of H₂O₂. Bacteria were treated for 30 minutes on each solution (0% to 10%) and serial dilutions were seeded on tryptic soy broth–defibrinated sheep blood (TSB-DSB) agar. Colonies were counted after five days of incubation. All treatments are effective in their bactericidal activity. a) Representative image of three independent experiments. b) Graph of percentage of viability of C. acnes versus percentage of H₂O₂ grown in TSB showed a MBC of 1% for H₂O₂ prepared in saline and in water. Topical solution is equally effective. d) C. acnes treated for 30 minutes with 3% H₂O₂ prepared in saline, in water, or 3% topical solution were grown on TSB-DSB agar as lawn to further assess for bacterial growth inhibition. None of the treatments produced growth of colonies, while controls saline and water only show normal bacterial growth.