Table II.

Mutations in the Liver cII Gene of Big Blue Mice Treated With AFB₁ as Neonates and Adults

Positiona	Mutationb	Amino acid change	Sequence context 5′ → 3′c	Number of mutationsn (number of independent mutations when different from mutants)
				Neonate	Adult
−14	G → T	N/A	ctaAGGaaa	1	1
−13	G → T	N/A	ctaAGGaaa	2
3	G → T	Met → Ile	catATGgtt	1
4	G → T	Val → Phe	atgGTTcgt	1
24/25	CG → AA	AsnGlu → LysLys	cgcAACGAGgct		1
25	G → A	Glu → Lys	aacGAGgct		1
	G → T	Glu → Stop	aacGAGgct	2
28	G → T	Ala → Ser	gagGCTcta		2
29	C → A	Ala → Asp	gagGCTcta		2(1)
34	C → T	Arg → Stop	ctaCGAatc		2
40	G → T	Glu → Stop	atcGAGagt	1	1
47	C → A	Ala → Glu	agtGCGttg		1
	C → G	Ala → Gly	agtGCGttg	1
51	G → T	Leu → Phe	gcgTTGctt	2(1)	1
64	G → A	Ala → Thr	atcGCAatg		3
73	G → T	Gly → Stop	cttGGAact	1	1
74	G → T	Gly → Val	cttGGAact		2
89	C → G	Ala → Gly	acaGCGgaa	1
	C → A	Ala → Glu	acaGCGgaa	8 (5)	3 (2)
	C → T	Ala → Val	acaGCGgaa	1	2
91	G → T	Glu → Stop	gcgGAAgct		1
94	G → C	Ala → Pro	gaaGCTgtg	1
95	C → A	Ala → Asp	gaaGCTgtg	1
101	G → T	Gly → Val	gtgGGCgtt		1
103	G → A	Val → Ile	ggcGTTgat		1
	G → T	Val → Phe	ggcGTTgat	5 (3)	4 (2)
108	T → A	Asp → Glu	gttGATaag		1
113	C → A	Ser → Stop	aagTCGcag		1
122	G → T	Ser → Ile	atcAGCagg	4 (3)
123	C → A	Ser → Arg	atcAGCagg	2	2
125	G → T	Arg → Met	agcAGGtgg	2
128	G → T	Trp → Leu	aggTGGaag	1
129	G → A	Trp → Stop	aggTGGaag	1
131	A → T	Lys → Met	tggAAGagg	1
134	G → T	Arg → Met	aagAGGgac	1
	G → C	Arg → Thr	aagAGGgac	1
135	G → T	Arg → Ser	aagAGGgac	1	1
135–136	GG → TT	ArgAsp → SerTry	aagAGGGACtgg	1
140	G → C	Trp → Ser	gacTGGatt		1
141	G → T	Trp → Cys	gacTGGatt	2(1)
145	C → A	Pro → Thr	attCCAaag	1	1
145–146	CC → AA	Pro → Lys	attCCAaag		1
145/146	−C	Frameshift	attCCAaag	1
150	G → T	Lys → Asn	ccaAAGttc	1
155	C → T	Ser → Leu	ttcTCAatg		1
158	−T	Frameshift	tcaATGctg		1
159	G → A	Met → Iie	tcaATGctg		1
159–160	GC → TG	MetLeu → IieVal	tcaATGCTGctt	1
160	C → A	Leu → Met	atgCTGctt	2	1
161	T → A	Leu → Gln	atgCTGctt		1
163	C → A	Leu → Ile	ctgCTTgct	2(1)
	C → T	Leu → Phe	ctgCTTgct	1
166	G → T	Ala → Ser	cttGCTgtt	2
167	C → A	Ala → Asp	cttGCTgtt	2
173/174	+GTTC	Frameshift	gttCTTgaa		1
178/185	+G	Frameshift	gaaTGGGGGGTCgtt	1
179	G → T	Trp → Leu	gaaTGGggg	1
179–184	−G	Frameshift	gaaTGGGGGGTCgtt		1
181	G → T	Gly → Trp	tggGGGgtc	2(1)
190	G → T	Asp → Tyr	gttGACgac	1
193	G → T	Asp → Tyr	gacGACgac	1	1
196	G → A	Asp → Asn	gacGACatg	1	1
	G → C	Asp → His	gacGACatg	1
	G → T	Asp → Tyr	gacGACatg		1
212	C → A	Ala → Glu	ttgGCGcga	18(6)	7 (4)
	C → T	Ala → Val	ttgGCGcga	3 (2)	5 (4)
214	C → T	Arg → Stop	gcgCGAcaa		2
217	C → A	Gln → Lys	cgaCAAgtt	1
224	C → A	Ala → Asp	gttGCTgcg	3
232	C → A	Leu → Ile	attCTCacc		1
256	−G	Frameshift	gcgGCAacc	1
285/286	−G	Frameshift	cagATGGAGttc		1
Total				93 (70)	63 (55)

−, deletion; +, insertion.

^aPosition 1 is the first base of the start codon in the cII coding sequence.

^bPresented in term of sequence change on nontranscribed DNA strand.

^cUppercase indicates target codon and target bases are underlined.