Table III.

RTG, MF and the percentage of small colonies induced by 11 CSCs

CSC	Dose (μg/ml)	Nicotine (μg/ml)a	Microwell version			Soft- agar version
			RTG (%)	MF (×10−6)	% of small colony	RTG (%)	MF (×10−6)	% of small colony
2R4F	0	0	100	76	50	100	50	43
	25	1.14	99	150	53	100	70	43
	50	2.28	68	231	62	63	129	64
	75	3.41	37	506	65	41	252	68
	100	4.55	19	972	60	21	451	82
	PC		43	874	53	42	387	62
LT	0	0	100	100	65	100	80	57
	30	1.73	76	221	66	86	126	68
	40	2.30	67	319	71	84	145	74
	50	2.88	44	617	70	48	271	80
	60	3.45	39	772	71	39	355	83
	70	4.03	27	1204	65	24	399	71
	PC		30	1167	64	43	349	65
LTMAS	0	0	100	69	40	100	70	54
	50	2.90	75	186	60	96	123	65
	75	4.35	54	334	59	65	260	76
	100	5.80	35	610	57	48	362	79
	125	7.25	17	1172	63	26	594	87
	PC		26	825	57	30	695	76
FF	0	0	100	88	26	100	87	33
	50	2.45	92	191	46	78	147	33
	75	3.68	72	279	50	67	159	47
	100	4.90	42	517	62	41	252	38
	125	6.13	29	581	64	26	301	51
	150	7.35	16	1003	70	16	420	54
	PC		64	952	52	62	356	37
LIP	0	0	100	87	29	100	67	39
	100	5.80	52	203	59	59	153	67
	125	7.25	47	324	56	44	284	68
	150	8.70	34	522	67	35	363	75
	175	10.15	23	761	60	22	544	75
	200	11.60	19	771	61	19	566	84
	PC		40	1290	48	39	664	72
LVB	0	0	100	100	65	100	80	57
	25	1.23	89	134	65	99	99	51
	50	2.45	79	199	68	71	111	54
	75	3.68	43	497	76	39	222	76
	100	4.90	14	1151	73	15	368	80
	PC		30	1167	64	43	349	65
LCF	0	0	100	80	36	100	62	26
	40	2.30	54	229	48	63	106	29
	60	3.45	50	276	63	45	133	54
	80	4.60	34	434	64	35	220	57
	100	5.75	13	772	67	17	277	70
	PC		55	832	50	52	373	38
BUR	0	0	100	93	33	100	87	32
	50	5.83	75	235	38	84	144	45
	75	8.74	47	239	53	48	157	52
	100	11.65	28	276	59	29	234	58
	125	14.56	20	612	68	25	260	72
	150	17.48	13	699	60	13	471	77
	PC		37	1396	39	56	398	76
BRI	0	0	100	96	35	100	53	30
	40	4.68	64	202	45	54	170	59
	50	5.85	37	444	62	39	245	73
	60	7.02	18	885	59	23	343	75
	70	8.19	11	996	60	12	467	81
	PC		45	999	40	41	482	61
REC	0	0	100	96	35	100	53	30
	40	1.28	58	255	52	56	157	56
	60	1.92	33	584	54	31	325	72
	70	2.24	17	1036	53	16	558	80
	80	2.56	12	1319	56	10	701	85
	PC		45	999	40	41	482	61
UL	0	0	100	93	33	100	87	32
	75	3.79	62	273	50	67	180	53
	100	5.05	45	344	50	46	254	65
	125	6.31	21	602	68	22	403	80
	150	7.58	14	892	66	16	470	75
	PC		37	1396	39	56	398	76

RTG, relative total growth that includes a measure of growth during treatment, expression and cloning; MF, mutant frequency per 10⁶ cells; PC, positive control of 0.3 μ/ml benzo[a]pyrene in each experiment.

^aConverted from CSC mass concentration according to the nicotine concentration in each CSC shown in Table I, in order to adjust the toxicity value for compensatory smoking.