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Hcy increases Cx43 phosphorylation on S368 (Cx43-pS368). (A) Cx43-pS368 (green) and total Cx43 (red) expression after treating with Hcy for the indicated times; control is secondary antibody only (omission of the primary antibody). Scale bar is 80 μm and applies to all images. (B-C) ELISA for the expression of Cx43 (B) and Cx43-pS368 (C) (n= 10). (D) Two hour treatments with Hcy and PMA, an activator of PKC, induce significantly greater expression of Cx43-pS368 (n = 10).
