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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Mol Microbiol. 2018 Jul 25;109(3):306–326. doi: 10.1111/mmi.13986

Figure 3.

Figure 3

TOEFAZ1 localization is altered in KPP1-depleted cells. A) KPP1 RNAi was induced using 1 μg/mL doxycycline in a cell line in which TOEFAZ1 was tagged at its endogenous locus with a Ty1 epitope tag. Lysates were collected every 24 h and subjected to western blotting using anti-Ty1 antibody. B) Cells from (A) were collected after four days, methanol-fixed, and stained using anti-Ty1 antibody and 1B41 antibody to label the FAZ. Arrowheads:Ty1-TOEFAZ1 localization. Asterisks indicate detached flagellar tips. DAPI was used to stain DNA. C) Quantification of TOEFAZ1 localization in cells from B).