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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Mol Microbiol. 2018 Jul 25;109(3):306–326. doi: 10.1111/mmi.13986

Figure 7.

Figure 7

KLIF is required for nascent posterior end formation and resolution of the cleavage furrow. A) Cells containing KLIF and TOEFAZ1 endogenously tagged with Ty1- and HA-epitope tags, respectively, were methanol-fixed and stained with anti-Ty1 and anti-HA antibody, as well as 1B41 to label the FAZ. Empty arrowheads: Ty1-KLIF. Filled-in arrowheads: HA-TOEFAZ1. B) Quantification of DNA state in control and KLIF RNAi cells that were PFA-fixed. C) The number of 2N2K and multinucleated cells containing cleavage furrows were counted in cells from B). To the right are representative images of stalled furrow formation (arrowheads). D) TOEFAZ1 RNAi was induced for 2 days in a cell line containing KLIF tagged at its endogenous locus with a Ty1 epitope tag. Cells were extracted using PEME- 0.25% NP-40, PFA-fixed, and stained with anti-Ty1 antibody. 1B41 was used to label the FAZ. Arrowhead points to Ty1-KLIF localization. In all immunofluorescence panels, cells were stained with DAPI for DNA.