Skip to main content
. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cancer Immunol Res. 2018 Dec 18;7(2):230–243. doi: 10.1158/2326-6066.CIR-18-0266

Figure 6: Cell Surface Screen of ADCCS1 and ADCCR1 Cells.

Figure 6:

A, Dot plot comparing geometric means of ADCCS1 and ADCCR1 cell surface molecule expression measured by the BD Lyoplate assay as described in Materials and Methods. Molecules with highest differential cell surface expression in ADCCS1 cells are shown in the box. BD lyoplate screen geometric means of ADCCS1 and ADCCR1 are in Supplementary Table S1. B, Geometric means of molecules with the highest differential expression (box in Fig. 6A) in ADCCS1 (blue) compared to ADCCR1 (red) cells. C, Representative histograms of selected cell surface molecules with reduced cell surface expression on ADCCR1 cells based on lower protein expression. Light gray histograms: negative control. Dark gray histograms: expression of total protein in permeabilized ADCCR1 cells. Open histograms: cell surface expression of indicated molecule in ADCCR1 cells. D, Representative histograms of selected cell surface molecules with reduced cell surface expression based on reduced transport to cell surface. Light gray histograms: negative control. Dark gray histograms: expression of total protein in permeabilized ADCCR1 cells. Open histograms: cell surface expression of indicated molecule in ADCCR1 cells.