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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Jan 3;127:232–245. doi: 10.1016/j.yjmcc.2018.12.016

Figure 2. LGM2605 corrects septic cardiac dysfunction in C57BL/6 mice following CLP surgery without reducing inflammatory cytokines.

Figure 2.

(A-B) Representative M-mode echocardiograms (A) and ejection fraction (EF), fractional shortening (FS), end diastolic volume, and end-systolic volume of C57BL/6 mice treated with LGM2605 6hrs post-CLP and monitored for 12hrs after the surgery (B). Sham: n=9, Sham+6hrs SDG: n=4, CLP: n=12, CLP+6hrs SDG: n=12, **P < 0.01 vs Sham, ###P < 0.001 vs CLP, $P < 0.05 vs Sham + LGM2605 by ANOVA with Bonferroni post-test. (C) LVdP/dtmax and (D) LVdP/dtmin in response to increasing doses of isoproterenol in mice that underwent sham surgery, CLP and combined CLP and LGM2605 treatment (6 h post-CLP), at 12 hrs timepoint. n=3 mice per group, **P < 0.01, ***P < 0.001 versus sham at corresponding timepoints, #P < 0.05 versus baseline, ##P < 0.01 versus baseline, ###P < 0.001 versus baseline, +P < 0.05 and ++ P < 0.01 versus 0.1ng isoproterenol, @ P<0.05 versus 0.5ng isoproterenol, by ANOVA with Bonferroni posttest. (E) Density of p adrenergic receptors using radio ligand binding assay, n=4–5 mice per group. ***P < 0.001 by ANOVA with Bonferroni post-test. (F) Immunoblotting and densitometric analysis of phosphorylated and total IkBa from ventricular tissue of mice 12 hours post-surgery. (G) Cardiac mRNA expression and (H) plasma levels of cytokines 12-hours post-surgery, n = 4–5 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA with Bonferroni post-test.