Figure 5.

1 and 18 promotes p16 and p27 production by blocking KDM5A-mediated H3K4me3 demethylation in MDA-MB-231 cells. Effect of 1 on the interactions KDM5A-H3K4me3 was examined by co-IP (A). Effect of 1 on H3K4me3 at promoters of p16 and p27 gene in MDA-MB-231 cells (B,C). ChIP assay was conducted with primary antibody against H3K4me3. transcriptional (D,E) and protein (F) levels of p21 and p27 in MDA-MB-231 cells treated with 1 or 18 were measured by Western blotting analysis. Bar graphs represented the mean enrichment relative to DMSO-treated groups and error bars reflect standard deviation of results derived from triplicate biological experiments. * p < 0.05, versus control (Student’s t-test).