Table 1. The characteristics of primers of candidate reference genes for RT-qPCR of Camellia sinensis.

Gene symbol	Accession number or Arabidopsis homolog locus	Forward/reverse primer sequence (5′–3′)	Amplicon length (bp)	Melting temperature (°C)	Efficiency value (%)	R2
Cs18SrRNA	AY563528.1	CGGCTACCACATCCAAGGAA/GCTGGAATTACCGCGGCT	191	63.2/63.5	95.6	0.994
CsGADPH	KA295375.1	TTGGCATCGTTGAGGGTCT/CAGTGGGAACACGGAAAGC	206	61.6/61.7	100.3	1.000
CsACT	KA280216.1	GCCATCTTTGATTGGAATGG/GGTGCCACAACCTTGATCTT	175	60.3/60.0	104.5	0.980
CsEF-1α	KA280301.1	TTCCAAGGATGGGCAGAC/TGGGACGAAGGGGATTTT	196	59.6/60.2	99.2	0.999
CsUbi	HM003234.1	GGAAGGACTTTGGCTGAC/GACCCATATCCCCAGAACAC	98	55.6/59.1	92.3	0.992
CsTUA	DQ444294.1	TCCAAACTAACCTTGTGCCATAC/ACACCCTTGGGTACTACATCTCC	220	60.3/60.5	97.5	0.983
Cs26SrRNA	AY283368	TCAAATTCCGAAGGTCTAAAG/CGGAAACGGCAAAAGTG	319	56.2/58.8	95.6	0.984
CsRuBP	EF011075.1	AAGCACAATTGGGAAAAGAAG/AAAGTGAAAATGAAAAGCGACAAT	405	58.4/60.4	103.4	0.999
CsCYP	AT3G56070	TTTGCGGATGAGAACTTCAA/CCATCTCCTTCACCACACTG	181	59.4/59.1	104.5	0.997
CselF-4α	AT3G13920	TGAGAAGGTTATGCGAGCAC/GCAACATGTCAAACACACGA	149	59.0/59.1	109.0	0.989
CsMON1	AT2G28390	ATTTCCTTCGTGGAGAATGG/GCCCATAAACAAGCTCCAAT	160	59.0/59.0	92.1	0.986
CsPCS1	AT5G44070	AATGCCCTTGCTATTGATCC/CTCCAGAACAGTGAGCCAAA	151	59.0/59.0	98.9	0.981
CsSAND	AT2G28390	GCCTGAACCGTCTTCTGTGGAGT/CTCAATCTCAGACACACTGGTGCTA	184	66.2/63.0	100.6	0.984
CsPP2A	AT3G21650	AAGAAGAGGAACTGGCGACGGAAC/CAAACAGGTCCAGCAAACGCAAC	153	67.9/68	95.7	0.993
CsTBP	AT1G55520	GGCGGATCAAGTGTTGGAAGGGAG/ACGCTTGGGATTGTATTCGGCATTA	166	68.0/68.1	97.6	0.987
CsLOX1	EU195885.2	AACAAGAACAACAATATATAGCTC/AAACGGAGCCTTCAACACC	165	51.8/60.1	101.1	0.994