Table 2. Characteristic features of wheat TaCKX (a), TaZOG (b), TaIPT (c) and TaGLU (d) gene families.
| Genes | Length (aa) | PI | MW (kDa) | Subcell location | Glyco. sites | |
|---|---|---|---|---|---|---|
| (a)Cytokinin oxidase/dehydrogenase (TaCKX) | ||||||
| 1 | TaCKX1 | 524 | 8.68 | 56.9 | ER & Vacuole | 5 |
| 2 | TaCKX2 | 555 | 6.18 | 59.8 | ER & Vacuole | 2 |
| 3 | TaCKX3 | 523 | 6.28 | 57.7 | ER & Vacuole | 0 |
| 4 | TaCKX4 | 527 | 6.53 | 57.8 | ER & Vacuole | 3 |
| 5 | TaCKX5 | 531 | 6.03 | 57.8 | ER & Vacuole | 2 |
| 6 | TaCKX7 | 535 | 8.49 | 58.5 | ER & Vacuole | 4 |
| 7 | TaCKX8 | 528 | 5.62 | 57.2 | ER & Vacuole | 0 |
| 8 | TaCKX9 | 521 | 6.86 | 58.3 | ER & Vacuole | 5 |
| 9 | TaCKX10 | 532 | 6.1 | 58.0 | ER & Vacuole | 3 |
| 10 | TaCKX11 | 516 | 5.93 | 55.7 | ER & Vacuole | 0 |
| 11 | TaCKX12 | 547 | 5.57 | 59.2 | ER & Vacuole | 1 |
| 12 | TaCKX13 | 545 | 6.05 | 58.8 | ER & Vacuole | 1 |
| 13 | TaCKX14 | 552 | 5.56 | 59.4 | ER & Vacuole | 1 |
| (b) Zeatin O-glucosyltransferases (TaZOG) | ||||||
| 1 | TaZOG1 | 491 | 5.99 | 53.4 | Plasma membrane | 2 |
| 2 | TaZOG2 | 481 | 5.74 | 53.1 | Secreted | 0 |
| 3 | TaZOG3 | 551 | 5 | 59.3 | Plasma membrane | 2 |
| 4 | TaZOG4 | 529 | 5.43 | 56.3 | Plasma membrane | 1 |
| 5 | TacisZOG1 | 467 | 5.87 | 50.8 | Secreted | 0 |
| 6 | TacisZOG3 | 466 | 6.23 | 50.6 | Secreted | 0 |
| 7 | TacisZOG4 | 528 | 6.93 | 57.5 | Secreted | 0 |
| (c) isopentenyl transferases (TaIPT) | ||||||
| 1 | TaIPT1 | 292 | 5.23 | 31.6 | chloroplast | 0 |
| 2 | TaIPT2 | 355 | 5.05 | 38.0 | chloroplast | 0 |
| 3 | TaIPT3 | 369 | 9.24 | 39.2 | chloroplast | 0 |
| 4 | TaIPT5 | 351 | 8.16 | 37.8 | chloroplast | 1 |
| 5 | TaIPT6 | 351 | 8.47 | 37.8 | chloroplast | 1 |
| 6 | TaIPT7 | 346 | 6.68 | 37.1 | chloroplast | 0 |
| 7 | TaIPT8 | 392 | 9.09 | 41.2 | chloroplast | 0 |
| 8 | TaIPT9 | 466 | 6.68 | 52.1 | Cytoplasm | 0 |
| 9 | TaIPT10 | 499 | 6.77 | 50.5 | Cytoplasm | 1 |
| (d) wheatβ-glucosidases (TaGLU) | ||||||
| Genes | Length (aa) | PI | MW (kDa) | subcell location | Glyco. sites | |
| 1 | TaGLU5 | 475 | 5.46 | 53.1 | vacuole | 4 |
| 2 | TaGLU6 | 427 | 7.15 | 49 | chloroplast | 2 |
| 3 | TaGLU7 | 508 | 9 | 56.6 | chloroplast | 1 |
| 4 | TaGLU8 | 585 | 6.72 | 64.6 | chloroplast | 2 |
| 5 | TaGLU9 | 532 | 6.9 | 59.5 | vacuole | 2 |
| 6 | TaGLU11 | 508 | 4.91 | 56.6 | vacuole | 3 |
| 7 | TaGLU12 | 519 | 6.93 | 58.6 | chloroplast | 4 |
| 8 | TaGLU13 | 508 | 5.7 | 57.5 | chloroplast | 1 |
| 9 | TaGLU14 | 519 | 6.79 | 59.5 | chloroplast | 2 |
| 10 | TaGLU15 | 430 | 5.35 | 48.4 | chloroplast | 1 |
| 11 | TaGLU16 | 511 | 6.12 | 57.8 | vacuole | 1 |
| 12 | TaGLU17 | 504 | 9.55 | 55.8 | chloroplast | 1 |
| 13 | TaGLU19 | 506 | 5.36 | 56.7 | vacuole | 2 |
| 14 | TaGLU21 | 473 | 5.4 | 52.4 | vacuole | 2 |
| 15 | TaGLU22 | 485 | 5.2 | 53.8 | vacuole | 2 |
| 16 | TaGLU23 | 477 | 5.6 | 53.4 | vacuole | 2 |
| 17 | TaGLU24 | 502 | 8.37 | 57.5 | chloroplast | 3 |
| 18 | TaGLU26 | 448 | 6.67 | 51.5 | chloroplast | 4 |
| 19 | TaGLU28 | 525 | 8.72 | 59.4 | chloroplast | 4 |
| 20 | TaGLU30 | 517 | 9.26 | 58 | vacuole | 3 |
| 21 | TaGLU31 | 503 | 6.05 | 56.5 | vacuole | 6 |
| 22 | TaGLU32 | 522 | 7.28 | 58.5 | vacuole | 1 |
| 23 | TaGLU34 | 515 | 6.92 | 58.4 | chloroplast | 4 |
| 24 | TaGLU35 | 406 | 6 | 46.1 | chloroplast | 2 |
| 25 | TaGLU38 | 502 | 7.29 | 58.4 | chloroplast | 5 |
Notes.
- PI
- Isoelectric point
- MW
- Molecular weight
- Glyco. Sites
- Glycosylation sites
- ER
- Endoplasmic reticulum
For TaGLU family, characteristic features of only newly identified genes are presented here.
PI & MW predicted by ExPASy (http://web.expasy.org/compute_pi/).
Subcell location predicted by Softberry (http://www.softberry.com/).
Glyco. Sites predicted by NetNGlyc (http://www.cbs.dtu.dk/services/NetNGlyc/).